L3771

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

• 동의어(들): Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
• Linear Formula: CH₃(CH₂)₁₁OSO₃Na
• CAS Number: 151-21-3
• Molecular Weight: 288.38
• Beilstein: 3599286
• EC Number: 205-788-1
• MDL number: MFCD00036175
• UNSPSC 코드: 12161902
• eCl@ss: 39093306
• PubChem Substance ID: 24896351
• NACRES: NB.22

속성

• 생물학적 소스: synthetic
• Quality Level: 200
• Grade: Molecular Biology; for molecular biology
• 설명: anionic
• 제품 라인: BioReagent
• 분석: ≥98.5% (GC)
• 양식: powder
• 분자량: micellar 18,000
• 응집 번호: 62
• 기술: electrophoresis: suitable
• 불순물: ≤2.0% water (Karl Fischer)
• CMC: 7-10 mM (20-25°C)
• mp: 204-207 °C (lit.)
• 전이 온도: cloud point >100 °C
• solubility: water: soluble
• density: 1.03 g/cm³ at 20 °C (68 °F)
• 음이온 미량물: chloride (Cl^-): ≤500 ppm; phosphate (PO₄^3-): ≤10 ppm
• 양이온 미량물: heavy metals (as Pb): ≤10 ppm
• 흡수: ≤0.1 at 260 at 3%; ≤0.1 at 280 at 3%
• HLB: 40
• 적합성: suitable for electrophoresis
• 외래 활성: DNase, RNase, none detected
• 저장 온도: room temp
• SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M

설명

일반 설명

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is an anionic surfactant widely used in various scientific applications, particularly in molecular biology, biochemical, and cell biology research. Its effectiveness stems from its amphiphilic nature, allowing it to interact with both hydrophobic and hydrophilic molecules. SDS is a versatile detergent that excels in solubilizing and denaturing proteins, making it an essential tool in protein extraction and purification procedures. By disrupting protein structures, SDS facilitates the separation of proteins based on their molecular weight during SDS-PAGE electrophoresis.

Beyond protein analysis, SDS also finds applications in the electrophoretic separation of lipids, enabling the study and characterization of these important cellular components. Additionally, SDS is employed in various hybridization and molecular biology techniques, demonstrating its versatility across a range of research areas. SDS′s effectiveness in both ionic and anionic conditions further expands its utility, allowing it to adapt to diverse experimental setups and requirements. Its ability to unravel proteins by binding to peptide chains and imparting a negative charge effectively eliminates protein shape as a factor for gel separation, ensuring accurate protein size determination.

애플리케이션

Anionic detergent

Sodium dodecyl sulfate has been used:

• in chromatin immunoprecipitation
• in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
• as a component of radioimmunoprecipitation assay buffer for immunoblotting

Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

특징 및 장점

• Highly versatile surfactant for your molecular biology and biochemical research
• Suitable for electrophoresis applications
• Tested to confirm low levels of heavy metal contamination,ensuring suitability for various applications.
  

분석 메모

Tested for use in denatured polyacrylamide gel electrophoresis.

기타 정보

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안전성 정보

• 픽토그램: GHS02,GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H228,H302 + H332,H315,H318,H335,H412
• 예방조치 성명서: P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 4.1B - Flammable solid hazardous materials
• WGK: WGK 2
• Flash Point (°F): 338.0 °F
• Flash Point (°C): 170 °C
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges