L4669

Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-LOX, Anti-Protein lysine 6-oxidase
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~50 kDa
• 종 반응성: human
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• 농도: ~1 mg/mL
• 기술: western blot: 1.5-3.0 mg/mL using HEK-293T cellls expressing human LOX
• UniProt 수납 번호: P28300
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... LOX(4015) ; mouse ... Lox(16948) ; rat ... Lox(24914)

설명

일반 설명

The LOX gene is located on the human chromosome at 5q23.1.

The gene LOX (lysyl oxidase) encodes a copper-dependent amine oxidase that belongs to LOX family of proteins. These proteins contain highly conserved C- terminal mature catalytic domains that include the copper binding site, the lysyl tyrosyl quinine (LTQ) cofactor residues, and the cytokine receptor like (CRL) domain. It is present in both intercellular and intracellular locations.

특이성

Anti-Lysyl Oxidase (C-terminal) specifically recognizes human lysyl oxidase (LOX).

애플리케이션

Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence staining
• immunohistochemistry

생화학적/생리학적 작용

Altered LOX gene expression and activity are linked to skin aging and senescence. Decreased LOX gene expression and activity have been associated with severe connective tissue disorders such as Ehler-Danlos syndrome, cutis laxa, and Menkes′ syndrome. Increased LOX gene expression are associated with the development of fibrotic diseases that involve connective tissue remodeling, such as atherosclerosis, scleroderma and liver cirrhosis. LOX protein also plays an important role in breast cancer metastasis.

LOX is implicated in various intracellular functions such as the regulation of cellular differentiation, cell migration and gene transcription.

The gene LOX (lysyl oxidase) encodes an extracellular matrix remodeling enzyme that catalyzes the oxidation of primary amino group of peptidyl lysine to reactive peptidyl aldehydes. It is mainly involved in the oxidation of lysine residues in elastin and collagen, resulting in the formation of covalent cross-linkages, which stabilize these fibrous proteins. It also plays a role in several cellular processes such as developmental regulation, tumor suppression, cell motility, and cellular senescence. It is involved in hypoxia-induced metastasis and serves as a therapeutic target for the treatment of metastases.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)