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Merck
모든 사진(1)

주요 문서

MAK120

Sigma-Aldrich

Urease Activity Assay Kit

sufficient for 100 colorimetric tests

동의어(들):

Urea Amidohydrolase Activity Assay Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric tests

응용 분야

environmental

검출 방법

colorimetric

관련 질환(들)

gastrointestinal diseases

저장 온도

2-8°C

일반 설명

Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in bacteria, yeast, and certain higher plant species. Urease activity is found in environmental and fecal samples.

애플리케이션

Urease Activity Assay Kit has been used to measure urease activity.

특징 및 장점

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

적합성

Suitable for the detection of Urease activity in biological and environmental samples.

원리

In this assay, urease catalyzes the hydrolysis of urea resulting in the production of ammonia. The ammonia is determined by the Berthelot method resulting in colorimetric product measure at 670 nm, proportionate to the urease activity present in the sample. One unit of urease is the amount of enzyme that catalyzes the formation of 1.0 μmole ammonia per minute at pH 7.0.

픽토그램

Health hazardCorrosionEnvironment

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

보충제 위험성

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

이미 열람한 고객

Bhawna Uprety et al.
Pharmaceutics, 14(1) (2022-01-22)
Tumour cells maintain a local hypoxic and acidic microenvironment which plays a crucial role in cancer progression and drug resistance. Urease is a metallohydrolases that catalyses the hydrolysis of urea into ammonia and carbon dioxide, causing an abrupt increase of
Ureases I. Functional, catalytic and kinetic properties: A review
Barbara K.
Journal of Molecular Catalysis. B, Enzymatic, 59(1-3), 9-21 (2009)
Chelsea M Heveran et al.
Scientific reports, 9(1), 14721-14721 (2019-10-13)
We demonstrate for the first time that the morphology and nanomechanical properties of calcium carbonate (CaCO3) can be tailored by modulating the precipitation kinetics of ureolytic microorganisms through genetic engineering. Many engineering applications employ microorganisms to produce CaCO3. However, control
Malassezia furfur promoting growth of Staphylococcus epidermidis by increasing pH when cultured in a lipid-free environment.
Yang Han et al.
Chinese medical journal, 132(7), 873-876 (2019-03-22)
Short-term treatment with the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) alters urea assimilation and modulates transcriptional profiles of genes involved in primary and secondary metabolism in maize seedlings.
Zanin L, et al.
Frontiers in Plant Science, 7, 845-845 (2016)

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