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Merck
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문서

U7752

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

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About This Item

CAS Number:
9002-13-5
효소 위원회 번호:
3.5.1.5 (BRENDA, IUBMB)
EC Number:
232-656-0
MDL number:
MFCD00070858
UNSPSC 코드:
12352204
eCl@ss:
32160410
NACRES:
NA.54

생물학적 소스

Canavalia ensiformis

Quality Level

200

형태

solid

분자량

hexamer 545 kDa
trimer ~272 kDa

포함

~20% dithiothreitol

적합성

suitable for marker for electrophoresis (non-denaturing PAGE)

저장 온도

−20°C

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일반 설명

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria. It belongs to the superfamily of amidohydrolases and phosphotriesterases. Urease is also found in leguminous seeds.

애플리케이션

Urease from Canavalia ensiformis (Jack bean) has been used:
  • as a component of the molecular mass standard in electrophoresis
  • as a component of the standard in size-exclusion chromatography
  • as a reference in native-polyacrylamide gel electrophoresis (PAGE)

생화학적/생리학적 작용

Urease is an essential enzyme of the nitrogen cycle. It plays a role in degrading urea into ammonia and carbamate. Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.

기타 정보

Contains approx. 1 mg protein

픽토그램

Health hazardExclamation mark
GHS08,GHS07

신호어

Danger

유해 및 위험 성명서

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

시험 성적서(COA)

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문서 라이브러리 방문

Anuradha Balasubramanian et al.
Journal of molecular biology, 400(3), 274-283 (2010-05-18)
Urease, a nickel-dependent metalloenzyme, is synthesized by plants, some bacteria, and fungi. It catalyzes the hydrolysis of urea into ammonia and carbon dioxide. Although the amino acid sequences of plant and bacterial ureases are closely related, some biological activities differ
Muhammad Ajmal Rana et al.
PloS one, 16(10), e0258568-e0258568 (2021-10-15)
Urea is the most popular and widely used nitrogenous fertilizer. High soil urease activity rapidly hydrolyses applied urea to ammonia which contributes to soil nitrogen (N) losses and reduces N use efficiency of crop plants. The ammonia losses can be
Paweł Kafarski et al.
Journal of advanced research, 13, 101-112 (2018-08-11)
Urease is a nickel-dependent metalloenzyme found in plants, some bacteria, and fungi. Bacterial enzyme is of special importance since it has been demonstrated as a potent virulence factor for some species. Especially it is central to Helicobacter pylori metabolism and
Suchithra Menon et al.
Cell, 156(4), 771-785 (2014-02-18)
mTORC1 promotes cell growth in response to nutrients and growth factors. Insulin activates mTORC1 through the PI3K-Akt pathway, which inhibits the TSC1-TSC2-TBC1D7 complex (the TSC complex) to turn on Rheb, an essential activator of mTORC1. However, the mechanistic basis of how
Prevention of infected urinary stones by urease inhibition.
D P Griffith et al.
Investigative urology, 11(3), 228-233 (1973-11-01)
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