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Merck
모든 사진(3)

문서

A8781

Sigma-Aldrich

β-Amylase from sweet potato

greener alternative

동의어(들):

1,4-α-D-Glucan maltohydrolase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
41106514
NACRES:
NA.56

형태

powder

Quality Level

분자량

~200,000

포장

vial of 15 mg

환경친화적 대안 제품 점수

old score: 57
new score: 1
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환경친화적 대안 제품 특성

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

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환경친화적 대안 카테고리

저장 온도

−20°C

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일반 설명

β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region. It belongs to the glycoside hydrolase family GH14.
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애플리케이션

β-Amylase from sweet potato has been used:
  • as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)
  • as a standard for calibration curve preparation in size exclusion chromatography
  • as a model protein for small-angle X-ray analysis (SAXs)

생화학적/생리학적 작용

β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends. Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


시험 성적서(COA)

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문서 라이브러리 방문

Luca Costa et al.
BMC structural biology, 16(1), 18-18 (2016-10-30)
Synchrotron radiation facilities are pillars of modern structural biology. Small-Angle X-ray scattering performed at synchrotron sources is often used to characterize the shape of biological macromolecules. A major challenge with high-energy X-ray beam on such macromolecules is the perturbation of
Khalilah G Reddie et al.
Molecular bioSystems, 4(6), 521-531 (2008-05-22)
Oxidation of the thiol functional group in cysteine (Cys-SH) to sulfenic (Cys-SOH), sulfinic (Cys-SO2H) and sulfonic acids (Cys-SO3H) is emerging as an important post-translational modification that can activate or deactivate the function of many proteins. Changes in thiol oxidation state
Integration of affinity precipitation with partitioning methods for bioseparation of chitin binding lectins.
Teotia, S. et al.
Food and Bioproducts Processing, 84, 37-43 (2006)
S Teotia et al.
Enzyme and microbial technology, 28(9-10), 792-795 (2001-06-09)
beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band
Liyu Tu et al.
Journal of cell science, 119(Pt 24), 5077-5086 (2006-12-13)
The surface of the mammalian urinary bladder is covered by a crystalline, asymmetric unit membrane (AUM) structure that contains the four major uroplakins (UPs): Ia, Ib, II and IIIa. UPIa and UPIb belong to the family of tetraspanins. Although UPIa

프로토콜

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.

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