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Merck
모든 사진(1)

주요 문서

MAK129

Sigma-Aldrich

β-Glucosidase Activity Assay Kit

sufficient for 100 colorimetric tests

동의어(들):

Beta-Glucosidase Assay Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric tests

검출 방법

colorimetric

관련 질환(들)

neurological disorders; gastrointestinal diseases; hematological disorder

저장 온도

−20°C

일반 설명

β-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing b(1→4)-linked D-glucose residues with the release of D-glucose. β-Glucosidases are required by organisms for the consumption of cellulose. Lysozyme, a β-glucosidase present in tears, acts on the β(1→4) glucose bonds present in the peptidoglycan cell wall of Gram-negative bacteria and helps to prevent bacterial infections in the eye. Defects in β-glucosidase activity have been implicated in Gaucher′s disease and Parkinson′s disease.

애플리케이션

β-Glucosidase Activity Assay Kit has been used to measure the β-glucosidase activity of the sample.

특징 및 장점

Compatible with high-throughput handling systems.

적합성

The β-Glucosidase Activity Assay kit provides a simple and direct procedure for measuring β-glucosidase activity in biological samples, for characterizing β-glucosidase production, and for the screening of β-glucosidase modulators.

원리

In this assay, β-glucosidase activity is determined by a reaction in which β-glucosidase hydrolyzes p-nitrophenyl-β-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the β-glucosidase activity present. One unit of β-Glucosidse is the amount of enzyme that catalyzes the hydrolysis of 1.0 μmole substrate per minute at pH 7.0.

Storage Class Code

12 - Non Combustible Liquids


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