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Merck
모든 사진(1)

문서

GUSS

Sigma-Aldrich

β-Glucuronidase Reporter Gene Staining Kit

동의어(들):

β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.32

사용

 kit sufficient for 100 histochemical assays

Quality Level

기술

microbe id | staining: suitable

배송 상태

dry ice

저장 온도

−20°C

일반 설명

Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.

애플리케이션

The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.

Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.

특징 및 장점

  • Histochemical staining of plant tissues expressing the E. coli GUS enzyme
  • Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
  • GUS does not interfere with plant cell function or viability

기타 정보

The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

키트 구성품 전용

제품 번호
설명

  • 2× Fixation Buffer 25 mL

  • 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial

  • Reagent A for gus staining kit 50 mL

  • Reagent B for gus staining kit 200 μL

  • Reagent C for gus staining kit 200 μL

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

이미 열람한 고객

Ken Naito et al.
Nature, 461(7267), 1130-1134 (2009-10-23)
High-copy-number transposable elements comprise the majority of eukaryotic genomes where they are major contributors to gene and genome evolution. However, it remains unclear how a host genome can survive a rapid burst of hundreds or thousands of insertions because such
Li-Ling Lin et al.
International journal of molecular sciences, 14(7), 14270-14286 (2013-07-11)
Brassinosteroids (BRs) are endogenous plant hormones and are essential for normal plant growth and development. MicroRNAs (miRNAs) of Arabidopsis thaliana are involved in mediating cell proliferation in leaves, stress tolerance, and root development. The specifics of BR mechanisms involving miRNAs
C-M Cheng et al.
Cancer gene therapy, 15(6), 393-401 (2008-03-29)
Increasing the specificity of chemotherapy may improve the efficacy of cancer treatment. Toward this aim, we developed a strain of bacteria to express enzymes for selective prodrug activation and non-invasive imaging in tumors. beta-glucuronidase and the luxCDABE gene cluster were
Kuo-Hsiang Chuang et al.
Bioconjugate chemistry, 17(3), 707-714 (2006-05-18)
Combination therapy can help overcome limitations in the treatment of heterogeneous tumors. In the current study, we examined whether multiple therapeutic agents could be targeted to anti-dansyl single-chain antibodies (DNS scFv) that were anchored on the plasma membrane of cancer
R A Jefferson et al.
The EMBO journal, 6(13), 3901-3907 (1987-12-20)
We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We

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