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Merck
모든 사진(2)

주요 문서

APOAF

Sigma-Aldrich

Annexin V-FITC Apoptosis Detection Kit

동의어(들):

Annexin V-FITC, Apoptosis probe FITC

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.84

사용

(20 tests)

Quality Level

포장

pkg of 1 kit

기술

flow cytometry: suitable

응용 분야

cell analysis
detection

검출 방법

fluorometric

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised. This combination allows the differentiation among early apoptotic cells (annexin V positive, PI negative), necrotic cells (annexin V positive, PI positive), and viable cells (annexin V negative, PI negative).

애플리케이션

Annexin V-FITC Apoptosis Detection Kit was used:
  • in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.
  • for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.
  • for indirect measurement of flippase activity.

특징 및 장점

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

기타 정보

Allow all components to reach room temperature before use.

키트 구성품 전용

제품 번호
설명

  • APOAFA

관련 제품

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

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문서 라이브러리 방문

Denise C Arruda et al.
The Journal of biological chemistry, 287(18), 14912-14922 (2012-02-16)
Complementarity-determining regions (CDRs) from monoclonal antibodies tested as synthetic peptides display anti-infective and antitumor activities, independent of the specificity of the native antibody. Previously, we have shown that the synthetic peptide C7H2, based on the heavy chain CDR 2 from
Jing-Fang Hong et al.
Molecular medicine reports, 13(6), 4541-4548 (2016-04-16)
The aim of the present study was to investigate the in vitro and in vivo anticancer and apoptotic effects of taraxerol acetate in U87 human glioblastoma cells. The effects on cell cycle phase distribution, cell cycle-associated proteins, autophagy, DNA fragmentation and cell migration were
J Dachary-Prigent et al.
Biochemistry, 34(36), 11625-11634 (1995-09-12)
The development of procoagulant activity and microparticle formation during platelet activation is known to depend on an increase in cytosolic Ca2+ levels. We have studied the mechanisms leading to these events using FITC-labeled recombinant annexin V, a protein which binds
Maria Camats et al.
PloS one, 3(8), e2926-e2926 (2008-08-14)
H-Ras pre-mRNA undergoes an alternative splicing process to render two proteins, namely p21 H-Ras and p19 H-Ras, due to either the exclusion or inclusion of the alternative intron D exon (IDX), respectively. p68 RNA helicase (p68) is known to reduce
Monika Kallubai et al.
Journal of biomolecular structure & dynamics, 37(3), 623-640 (2018-01-30)
Our study focus on the biological importance of synthesized 5β-dihydrocortisol (Dhc) and 5β-dihydrocortisol acetate (DhcA) molecules, the cytotoxic study was performed on breast cancer cell line (MCF-7) normal human embryonic kidney cell line (HEK293), the IC50 values for MCF-7 cells

문서

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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