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Merck
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문서

N2635

Sigma-Aldrich

Nucleoside 5′-Diphosphate Kinase from bovine liver

buffered aqueous glycerol solution, ≥1,000 units/mg protein (biuret)

동의어(들):

NDK, UDPkinase, nonmetastatic23(NM23), uridine diphosphate kinase, ATP:nucleoside diphosphate phosphotransferase, NDPK

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About This Item

CAS Number:
9026-51-1
효소 위원회 번호:
2.7.4.6 (BRENDA, IUBMB)
MDL number:
MFCD00131734
UNSPSC 코드:
12352204
eCl@ss:
32160410
NACRES:
NA.54

생물학적 소스

bovine liver

형태

buffered aqueous glycerol solution

특이 활성도

≥1,000 units/mg protein (biuret)

저장 조건

(Tightly closed)

기술

activity assay: suitable

외래 활성

lactic dehydrogenase, myokinase, β-NADH oxidase, nucleoside monophosphokinase and ATPase ≤0.1%

저장 온도

2-8°C

일반 설명

Research area: Cell Signaling

Nucleoside 5′-Diphosphate Kinase (NDK) is a ubiquitous housekeeping enzyme. Nucleoside Diphosphate Kinase exists in two isoforms in eukaryotic cells, NDK-A and NDK-B. These enzymes are found expressed both in the mitochondria and the cytoplasm.

애플리케이션

Nucleoside 5′-Diphosphate Kinase has been used:
  • nucleoside diphosphate kinase (NDPK) assays
  • coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP)
  • non-metastatic protein 23 (NM23) growth stimulation assay
  • in a study to assess inhibition of type I Fc epsilon receptor mediated Ca2+ influx and mediator secretion in rat mucosal mast cells
  • in a study to investigate protein synthesis in rabbit reticulocytes

생화학적/생리학적 작용

Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs). NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization.

단위 정의

One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.

관련 제품

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기질

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항체

제품 번호
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효소

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

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