N2876

Neuraminidase from Clostridium perfringens (C. welchii)

Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder

• 동의어(들): Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
• CAS Number: 9001-67-6
• 효소 위원회 번호: 3.2.1.18 (BRENDA, IUBMB)
• EC Number: 232-624-6
• MDL number: MFCD00131711
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• Quality Level: 300
• 유형: Type V
• 양식: lyophilized powder
• 특이 활성도: ≥0.1 units/mg solid (using mucin); ≥1.3 units/mg solid (using 4MU-NANA)
• 응용 분야: diagnostic assay manufacturing
• 외래 활성: Protease and NAN-aldolase, present
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: Clostridium perfringens str. 13 ... nanI(988807)

설명

일반 설명

Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.

애플리케이션

Neuraminidase from Clostridium perfringens has been used in a study to assess binding with human T lymphocytes in sheep pretreated with neuraminidase. It has also been used in a study to investigate the effect of bile salts on the action of hydrolysis by neuraminidase.

생화학적/생리학적 작용

Neuraminidase can increase aggregation in certain cell lines by removing exposed negatively charged sialic acid residues on the cell surface.

Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods.

Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues.

The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C.

단위 정의

One unit will liberate 1.0 micromole of N-acetyl neuraminic acid per minute at pH 5.0 at 37 °C using bovine submaxillary mucin.

One unit will hydrolyze 1.0 micromole of 2′-(4-methylumbelliferyl)-a-D-N-actetylneuraminic acid per minute at pH 5.0 at 37 °C (using 4MU-NANA as a substrate)

제조 메모

Prepared by salt fractionation.

분석 메모

Package sizes based on 4MU-NANA units

Package sizes based on the 4MU-NANA units

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)