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Merck
모든 사진(1)

주요 문서

N6514

Sigma-Aldrich

Neuraminidase from Vibrio cholerae

Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)

동의어(들):

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

Vibrio cholerae

Quality Level

유형

Type II

양식

buffered aqueous solution

특이 활성도

8-24 units/mg protein (Lowry, using NAN-lactose)

외래 활성

Protease and NAN-aldolase, present

저장 온도

2-8°C

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일반 설명

Calorimetry studies show that the two lectin-like domains flanking the central catalytic domain of Neuraminidase serve as the recognition and binding sites for sialic acid-containing substrates.
Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.

애플리케이션

Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells.
Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

품질

Preservative free.

단위 정의

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

물리적 형태

Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride, 0.2 μm filtered..

제조 메모

A further purification by affinity chromatography of our Type III.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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시험 성적서(COA)

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문서 라이브러리 방문

S Crennell et al.
Structure (London, England : 1993), 2(6), 535-544 (1994-06-15)
Vibrio cholerae neuraminidase is part of a mucinase complex which may function in pathogenesis by degrading the mucin layer of the gastrointestinal tract. The neuraminidase, which has been the target of extensive inhibitor studies, plays a subtle role in the
Purification and Properties of Neuraminidase from Vibrio cholera
Ada, G., et al.
Microbiology, 24, 409-421 (1961)
Nicolas Burin des Roziers et al.
Transfusion, 55(6 Pt 2), 1563-1571 (2015-01-06)
Pneumococcal hemolytic uremic syndrome (P-HUS) is a rare but severe complication of invasive pneumococcal disease (IPD) in young children. Consensual biologic diagnosis criteria are currently lacking. A prospective study was conducted on 10 children with culture-confirmed IPD. Five presented with
Hirokazu Kai et al.
Bioorganic & medicinal chemistry, 20(8), 2739-2746 (2012-03-14)
A selective and potent inhibitor of neuraminidases, a hydrolase that is responsible for processing sialylated glycoconjugates, is a promising drug candidate for various infective diseases. The current study demonstrates that the use of an aglycone-focused library of 2-difluoromethylphenyl α-sialosides is
Paul Clark et al.
Viruses, 12(10) (2020-10-24)
Polyomaviruses are small, non-enveloped DNA tumor viruses that cause serious disease in immunosuppressed people, including progressive multifocal leukoencephalopathy (PML) in patients infected with JC polyomavirus, but the molecular events mediating polyomavirus entry are poorly understood. Through genetic knockdown approaches, we

문서

Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

프로토콜

Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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