O4264

Human Osteopontin

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• 동의어(들): Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• MDL number: MFCD03100698
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• product name: Osteopontin human, recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in NSO cells
• 무균: sterile
• 분석: ≥95% (SDS-PAGE)
• 형태: lyophilized powder
• 분자량: 60-65 kDa by SDS-PAGE (reducing)
• 포장: pkg of 50 μg
• 기술: cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• UniProt 수납 번호: P10451
• 배송 상태: ambient
• 저장 온도: −20°C
• 유전자 정보: human ... SPP1(6696)

설명

일반 설명

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.

애플리케이션

Anti-Osteopontin antibody produced in rabbit has been used for ELISA.

생화학적/생리학적 작용

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

분석 메모

The biological activity is measured by its ability to mediate 293 cell adhesion.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)