P1869

Anti-Phosphotyrosine antibody, Mouse monoclonal

clone pT-154, purified from hybridoma cell culture

• 동의어(들): Phospho-Tyr, Phospho-tyrosine, p-Tyr
• MDL number: MFCD00164715
• UNSPSC 코드: 12352203
• NACRES: NA.44

속성

• 결합: unconjugated
• Quality Level: 200
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: pT-154, monoclonal
• 농도: ~2 mg/mL
• 기술: direct ELISA: suitable; immunohistochemistry: suitable; microarray: suitable; western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF
• 동형: IgG2b
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Anti-Phosphotyrosine antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the pT-154 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a tyrosine phosphorylated peptide. Members of protein-tyrosine kinases (PTKs) family consists of extra-cellular domain that is responsible for ligand binding, a trans-membrane domain, modular domain and an intracellular domain.

면역원

tyrosine phosphorylated peptide.

애플리케이션

Anti-Phosphotyrosine antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• western blot
• immunohistochemistry

생화학적/생리학적 작용

Protein phosphorylation is a post-translational modification that regulates signal transduction. Phosphorylation of tyrosine residues in proteins has been associated with oncogenesis, cell growth and survival . Monoclonal Anti-Phosphotyrosine antibody is specific for rat and human proteins/peptides that contain phosphotyrosine residues. As determined by competitive ELISA, the product does not bind to non-phosphorylated tyrosine, phosphorylated serine or threonine, ATP or AMP.

Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of Γ-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis and development. They are tightly regulated by other kinases and by autophosphorylation activity. Monoclonal antibodies specific for phosphotyrosine are essential tools for the study of tyrosine phosphorylation in post-translational modification in many biological processes.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable