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Merck
모든 사진(3)

문서

P3627

Sigma-Aldrich

Phosphatase, Acid from wheat germ

≥0.4 unit/mg solid

동의어(들):

(Orthophosphoric-monoester phosphohydrolase [acid optimum] ), Acid Phosphatase from wheat germ

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
eCl@ss:
42010102
NACRES:
NA.54

형태

powder

특이 활성도

≥0.4 unit/mg solid

분자량

58 kDa by gel filtration

저장 온도

−20°C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Product P3627 is from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.

생화학적/생리학적 작용

Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.

품질

Contains lipase

단위 정의

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

제조 메모

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서 라이브러리 방문

H G Yu et al.
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FEBS letters, 554(1-2), 50-54 (2003-11-05)
Phospholipase D (PLD) forms the major family of phospholipases that was first discovered and cloned in plants. In this report we have shown, for the first time, that C2 phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea associated
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프로토콜

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

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