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Merck
모든 사진(5)

주요 문서

P4020

Sigma-Aldrich

Protamine sulfate

amorphous powder

동의어(들):

Salmine, Salmine from salmon

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.77

제품명

Protamine sulfate salt from salmon, Grade X, amorphous powder

유형

Grade X

Quality Level

양식

amorphous powder

불순물

histone, free (Millon test)

색상

white to off-white

solubility

6 M HCl: 25 mg/mL

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일반 설명

Protamine are proteins rich in cysteine and arginine. It possesses many phosphorylation sites. It is found to be localized to the sperm head.

애플리케이션

Protamine sulfate salt from salmon has been used:
  • in the pretreament of cells to study its effect on respiratory syncytial virus infection
  • in lentivirus production
  • to derivatize it into a diazeniumdiolate NO donor

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

생화학적/생리학적 작용

Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

이미 열람한 고객

The design and evaluation of heparin-binding foldamers.
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Angewandte Chemie (International ed. in English), 44(41), 6685-6689 (2005-08-12)
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Bailey SN, et al.
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Nature methods, 3(2), 117-122 (2006-01-25)
Here we describe lentivirus-infected cell microarrays for the high-throughput screening of gene function in mammalian cells. To create these arrays, we cultured mammalian cells on glass slides 'printed' with lentiviruses pseudotyped as vesicular stomatitis virus glycoprotein, which encode short hairpin

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