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Merck
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P6611

Millipore

HIS-Select® Nickel Affinity Gel

(1:1 suspension in a 20% ethanol solution)

동의어(들):

Ni-NTA resin, nickel charged agarose

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모든 사진(2)

About This Item

UNSPSC 코드:
12352200
NACRES:
NA.56

결합

magnetic beads

Quality Level

200

형태

(1:1 suspension in a 20% ethanol solution)

특징

hydrophilic

포장

pkg of 1 mL
pkg of 100 mL
pkg of 25 mL
pkg of 5 mL
pkg of 500 mL

농도

1.5-2.4 mL/mL (suspension in packed gel)

기술

protein purification: suitable

색상

faint blue to very dark blue

기질

6% Beaded Agarose

용량

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

전이 온도

flash point 32 °C (closed cup)

저장 온도

2-8°C

관련 카테고리

일반 설명

HIS-Select® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.
HIS-Select® Nickel Affinity Gel is durable and can capture the recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with histidine tags are bound using either native or denaturing conditions.

애플리케이션

HIS-Select® Nickel Affinity Gel has been used in the purification of recombinant proteins like EF-hand calcium-binding protein (S100A14), LIM homeobox transcription factor 1 alpha protein, sigma-1 receptor as well as harpin, stable protein 1 (SP1), and BCR-ABL fusion protein.

특징 및 장점

  • High selectivity for higher purity.
  • Unique non-charged hydrophilic linkage reduces non-specific binding.
  • Binding capacity for histidine-tagged protein is greater than 15 mg/mL.
  • Binding under denaturing or non-denaturing conditions.
  • One-step purification.
  • Minimizes unwanted ionic interactions.
  • Minimal nickel leaching.
  • Bead size: 45-165 μm.

결합

It is also available with the EZview™ technology (Product Code E3528).

물리적 형태

1:1 suspension in a 20% ethanol solution

저장 및 안정성

HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer.

법적 정보

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point (°F)

89.6 °F - closed cup

Flash Point (°C)

32 °C - closed cup

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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