P8833

Puromycin dihydrochloride from Streptomyces alboniger

powder, non-animal origin, suitable for cell culture, BioReagent

• 동의어(들): 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride
• 실험식(Hill 표기법): C₂₂H₂₉N₇O₅ · 2HCl
• CAS Number: 58-58-2
• Molecular Weight: 544.43
• Beilstein: 3853613
• EC Number: 200-387-8
• MDL number: MFCD00012691
• UNSPSC 코드: 12352207
• PubChem Substance ID: 24898984
• NACRES: NA.76

속성

• product name: Puromycin dihydrochloride from Streptomyces alboniger, powder, BioReagent, suitable for cell culture
• 생물학적 소스: Streptomyces alboniger
• Quality Level: 300
• 제품 라인: BioReagent
• 분석: ≥98% (HPLC)
• 형태: powder
• 분자량: 544.43 g/mol
• 포장: pkg of 10 mg; pkg of 100 mg; pkg of 25 mg
• 환경친화적 대안 제품 점수: old score: 88; new score: 79; Find out more about DOZN™ Scoring
• 환경친화적 대안 제품 특성: Atom Economy; Design for Energy Efficiency; Use of Renewable Feedstocks; Design for Degradation; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 기술: cell culture | mammalian: suitable
• 색상: yellow-white
• UniProt 수납 번호: P13249
• 항생제 활성 스펙트럼: Gram-positive bacteria; neoplastics; parasites
• 응용 분야: agriculture
• 환경친화적 대안 카테고리: Re-engineered,
• 동작 모드: protein synthesis | interferes
• 저장 온도: −20°C
• SMILES string: Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1
• InChI: 1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1
• InChI key: MKSVFGKWZLUTTO-FZFAUISWSA-N

설명

일반 설명

Chemical structure: peptidyl nucleoside

Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger. It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes.

We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Atom Economy”, “Design for Energy Efficiency”, “Use of Renewable Feedstock” and “Design for Degradation”. Click here to view its DOZN scorecard.

애플리케이션

Puromycin dihydrochloride from Streptomyces alboniger has been used:

• in the preparation of puromycin stock solution for puromycin sensitivity assay.
• to select lentivirus-infected cells containing puromycin resistant selection marker.

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

Used as a selective agent for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). Recommended for use at a range of 1-10 μg/ml. Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.

생화학적/생리학적 작용

Puromycin associates in a non-specific manner with growing polypeptide chains, and thus, leads to premature termination of translation. It inhibits protein synthesis in two ways. It associates with the donor substrate, peptidyl-tRNA, in the P site and thus, functions as an acceptor substrate. Second, it competes with aminoacyl tRNA to bind with the A′ site within the peptidyl transferase center.

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

주의사항

As supplied, this product remains active for four years when stored at -20°C.

제조 메모

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

기타 정보

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302
• 예방조치 성명서: P301 + P312 + P330
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves