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Merck
모든 사진(1)

주요 문서

P9172

Sigma-Aldrich

Pyronin Y

for NA electrophoresis

동의어(들):

Pyronin Y, Pyronin G, Pyronin J

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About This Item

실험식(Hill 표기법):
C17H19ClN2O
CAS Number:
Molecular Weight:
302.80
색상 지수 번호:
45005
Beilstein:
3795789
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.52

Grade

for molecular biology

Quality Level

양식

powder

구성

Dye content, ≥45%

기술

nucleic acid detection: suitable

mp

250-260 °C (lit.)

solubility

water: 10 mg/mL, dark red

적합성

suitable for nucleic acid staining

저장 온도

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

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애플리케이션

Pyronin Y has been used:
  • in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
  • as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
  • staining of CD34+ cells

생화학적/생리학적 작용

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

제조 메모

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Transcriptional profiling of mammary gland side population cells
Behbod F, et al.
Stem Cells, 24(4), 1065-1074 (2006)
Cristina Mesa-Núñez et al.
Stem cell research & therapy, 11(1), 164-164 (2020-04-30)
CD18 is the common beta subunit of β2 integrins, which are expressed on hematopoietic cells. β2 integrins are essential for cell adhesion and leukocyte trafficking. Here we have analyzed the expression of CD18 in different subsets of human hematopoietic stem
Staining of RNA after polyacrylamide gel electrophoresis.
K Marcinka
Methods in cell biology, 10, 49-68 (1975-01-01)
Green, F.J., ed.
The Sigma-Aldrich Handbook of Stains, Dyes and Indicators,, 601-602 (1990)
A gel electrophoresis method for determining the relative binding constants of biologically active, intercalating fluorescent stains.
S M Hassur et al.
Analytical biochemistry, 95(2), 329-339 (1979-06-01)

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