추천 제품
재조합
expressed in E. coli
Quality Level
분석
≥95% (SDS-PAGE)
양식
lyophilized powder
포장
pkg of 1 kit (4 components)
배송 상태
wet ice
저장 온도
−20°C
일반 설명
Recombinant PURedit Cas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, Streptococcus pyogenes PURedit Cas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
PURedit translational-grade CRISPR reagents are engineered to meet superior manufacturing standards that enable consistent, high-quality performance every time.
PURedit translational-grade CRISPR reagents are engineered to meet superior manufacturing standards that enable consistent, high-quality performance every time.
애플리케이션
Functional Genomics; Target Validation; Genome Editing
특징 및 장점
- High Quality for preclinical and early clinical research
- Highest performance efficiency of any Cas9 variant
- Ready to inject/transfect
포장
pkg of 50 μg (≥ 300 pmol)
pkg of 250 μg (≥ 1500 pmol)
pkg of 250 μg (≥ 1500 pmol)
성분
Each kit consists of:
- one vial of PURedit Cas9 Recombinant Protein
- one vial of PURedit Transfection Buffer
- one vial of Reconstitution Buffer
- one vial of Dilution Buffer
원리
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
재구성
Lyophilized PURedit Cas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.
기타 정보
Use our CRISPR Selection Tool to order gRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
법적 정보
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
PURedit is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
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시험 성적서(COA)
프로토콜
Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.