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Merck
모든 사진(1)

주요 문서

PECAS9

Sigma-Aldrich

PURedit® Cas9 Protein

from Streptococcus pyogenes, recombinant, expressed in E. coli, 3X NLS

동의어(들):

Cas9, SpCas9, SpyCas9

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.51

재조합

expressed in E. coli

Quality Level

분석

≥95% (SDS-PAGE)

양식

lyophilized powder

포장

pkg of 1 kit (4 components)

배송 상태

wet ice

저장 온도

−20°C

일반 설명

Recombinant PURedit Cas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, Streptococcus pyogenes PURedit Cas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.

PURedit translational-grade CRISPR reagents are engineered to meet superior manufacturing standards that enable consistent, high-quality performance every time.

애플리케이션

Functional Genomics; Target Validation; Genome Editing

특징 및 장점

  • High Quality for preclinical and early clinical research
  • Highest performance efficiency of any Cas9 variant
  • Ready to inject/transfect

포장

pkg of 50 μg (≥ 300 pmol)
pkg of 250 μg (≥ 1500 pmol)

성분

Each kit consists of:
  • one vial of PURedit Cas9 Recombinant Protein
  • one vial of PURedit Transfection Buffer
  • one vial of Reconstitution Buffer
  • one vial of Dilution Buffer

원리

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

재구성

Lyophilized PURedit Cas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.

기타 정보

Use our CRISPR Selection Tool to order gRNA

Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins

법적 정보

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
PURedit is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

프로토콜

Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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