TRACRRNA05N

SygRNA^™ Cas9 Synthetic tracrRNA

SygRNA^™ SpCas9 tracrRNA

• 동의어(들): RNA scaffold
• UNSPSC 코드: 41106609
• NACRES: NA.51

속성

• 포장: pkg of 5 nmol × Tube (HPLC)
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

The SygRNA^™ synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA^™ synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation.

애플리케이션

Functional Genomics/Transgenic Applications

• Creation of gene knockouts
• Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes

특징 및 장점

HPLC-purified SygRNA^™ Cas9 tracrRNA oligo is optimized for use with a target-specific cRNA and a source of SpCas9 protein for genome editing applications.

결합

The SygRNA^™ Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein such as TGEN-CP-50UG, CAS9P, or CAS9MRNA to form active RNP complexes. Select predesigned unique crRNAs against human, mouse and rat genes at CRISPR search.

Learn more about SygRNA^™ tracrRNA

Need custom tracrRNA ?

물리적 형태

Dry

제조 메모

Resuspend SygRNA^™ Cas9 tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.

법적 정보

CRISPR Use License Agreement

SygRNA is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable