추천 제품
생물학적 소스
mouse
Quality Level
결합
peroxidase conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
secondary antibodies
클론
RabT-50, monoclonal
형태
buffered aqueous solution
종 반응성
rabbit
반응하면 안 됨
human, horse, bovine, cat, rat, chicken, pig, goat, monkey
농도
2.5-5.0 mg/mL (antibody)
기술
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody
western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.
동형
IgG1
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
일반 설명
Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).
Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies.
특이성
Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.
면역원
Enriched fraction of rabbit IgG light chain.
애플리케이션
Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- western blotting
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A novel Cep120-dependent mechanism inhibits centriole maturation in quiescent cells
Betleja E, et al.
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