R9782

RS-1

≥98% (HPLC)

• 동의어(들): 3-[(benzylamino)sulfonyl]-4-bromo-N-(4-bromophenyl)benzamide, 4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]-benzamide, RAD51-stimulatory compound 1
• 실험식(Hill 표기법): C₂₀H₁₆Br₂N₂O₃S
• CAS Number: 312756-74-4
• Molecular Weight: 524.23
• MDL number: MFCD00348720
• UNSPSC 코드: 12352200
• PubChem Substance ID: 329824048
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥98% (HPLC)
• 양식: powder
• 색상: off-white to light tan
• solubility: DMSO: ≥10 mg/mL
• 저장 온도: room temp
• SMILES string: Brc1ccc(NC(=O)c2ccc(Br)c(c2)S(=O)(=O)NCc3ccccc3)cc1
• InChI: 1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)
• InChI key: SWKAVEUTKGKHSR-UHFFFAOYSA-N

설명

애플리케이션

RS-1 has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.

RS-1 has been used:

• as a homology-directed repair (HDR) agonist to study its effects on in human hematopoietic stem/progenitor cells (HSPCs)
• as HDR agonist to analyze its effects on DNA repair modulation in human induced pluripotent stem (iPS) cells
• as RAD51 agonist to study its effects on double-stranded break repair

생화학적/생리학적 작용

RS-1 (RAD51-stimulatory compound 1) is a stimulator of the human homologous recombination (HR) protein RAD51. RS-1 stimulates binding of hRAD51 to single stranded DNA (ssDNA) and enhances recombinogenic activity by stabilizing the active form of hRAD51 filaments without inhibiting hRAD51 ATPase activity. RS-1 has been shown to enhance CRISPR-Cas9 knock-in efficiency in HEK293A cells and has been shown to enhance both TALEN and Cas9-mediated knock-in efficiency in rabbits.

RS-1 is a sulfonamido-benzamide compound.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable