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Key Documents

S100HR

Sigma-Aldrich

Sephacryl®

100-HR, MW range 1000-100,000 Da (globular proteins)

동의어(들):

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
23151817
NACRES:
NA.56

설명

stability: stable to 0.2M NaOH

형태

suspension (20% aqueous ethanol)

특징

autoclavable

기질

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

입자 크기

25-75 μm (wet)
25-75 μm (wet)

공극 크기

1000-100,000 Da MW range (globular proteins)

호환성

mode of use size exclusion chromatography

저장 온도

2-8°C

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일반 설명

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)

애플리케이션

Sephacryl® has been used:
  • to develop a novel high-throughput screening assay for sickle cell disease drug discovery
  • to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

법적 정보

Sephacryl is a registered trademark of Cytiva

관련 제품

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point (°F)

100.4 - 109.4 °F - closed cup

Flash Point (°C)

38 - 43 °C - closed cup

개인 보호 장비

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

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Clinica chimica acta; international journal of clinical chemistry, 411(3-4), 190-197 (2009-11-11)
Pseudomonas aeruginosa is an opportunistic pathogen that causes disease in immunocompromised individuals, burn victims, and cystic fibrosis patients. Strains that secrete ExoU induce host cell lysis and damage epithelial tissue, which can lead to severe outcomes including sepsis and mortality.
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The Journal of cell biology, 167(2), 315-325 (2004-10-27)
We purified native Myo2p/Cdc4p/Rlc1p (Myo2), the myosin-II motor required for cytokinesis by Schizosaccharomyces pombe. The Myo2p heavy chain associates with two light chains, Cdc4p and Rlc1p. Although crude Myo2 supported gliding motility of actin filaments in vitro, purified Myo2 lacked
David Novak et al.
Bioelectrochemistry (Amsterdam, Netherlands), 119, 136-141 (2017-10-11)
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Journal of bacteriology, 190(8), 3076-3082 (2008-02-12)
Bacteria can reduce toxic and carcinogenic Cr(VI) to insoluble and less toxic Cr(III). Thermus scotoductus SA-01, a South African gold mine isolate, has been shown to be able to reduce a variety of metals, including Cr(VI). Here we report the
B H Kang et al.
Archives of biochemistry and biophysics, 263(2), 387-393 (1988-06-01)
A simple procedure using ammonium sulfate to fractionate water-soluble cytochromes c553 and c550 on Sephacryl S-200 gel is described. The usefulness of this procedure has been studied using the crude extracts of mesophilic cyanobacteria. It was found that almost all

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