추천 제품
무균
sterile-filtered
Quality Level
제품 라인
BioReagent
형태
liquid
저장 조건
protect from light
기술
cell culture | mammalian: suitable
불순물
endotoxin, tested
배송 상태
ambient
저장 온도
2-8°C
애플리케이션
For suspension cell cultures
Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.
INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.
TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.
SELENIUM is an essential trace element normally provided by Serum.
SODIUM PYRUVATE has been shown to be beneficial as an additional energy source in some instances.
ETHANOLAMINE is a fatty acid that plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.
Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.
TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.
SELENIUM is an essential trace element normally provided by Serum.
SODIUM PYRUVATE has been shown to be beneficial as an additional energy source in some instances.
ETHANOLAMINE is a fatty acid that plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.
Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
성분
Contains 1.0mg/ml recombinant human insulin, 0.55mg/ml human transferrin (substantially iron-free), 0.5μg/ml sodium selenite, 12mg/ml pyruvate and 0.2mg/ml ethanolamine at the 100x concentration.
기타 정보
SPITE is a mixture of recombinant human insulin, human transferrin (partially iron-saturated), sodium selenite, sodium pyruvate and ethanolamine. It is designed for cell cultures in which media without sodium pyruvate are used.
제조 메모
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.
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