추천 제품
무균
sterile-filtered
Quality Level
제품 라인
BioReagent
형태
liquid
기술
cell culture | mammalian: suitable
불순물
endotoxin, tested
배송 상태
ambient
저장 온도
2-8°C
일반 설명
SITE Liquid Media Supplement (100×) serves as a substitute for serum-free formulations. It contains purified factors required for in vitro growth and differentiation studies. The addition of supplements to media will vary, depending on the cell type being studied and the basal medium employed. It is a general cell supplement designed for use in non-complex media (e.g., minimum essential medium (MEM), Roswell Park Memorial Institute Medium (RPMI-1640)) and complex media (e.g., Ham′s F-12, Dulbecco′s Modified Eagle Medium (DME) /F-12, MEM) with sodium pyruvate.
애플리케이션
SITE Liquid Media Supplement (100×) has been used:
- in the 3D culture of mouse embryonic pancreatic epithelial cells
- to isolate rabbit gastric glands and parietal cells
- to isolate rabbit single proximal tubule cells
생화학적/생리학적 작용
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
- Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
- Selenium: an essential trace element normally provided by serum.
- Ethanolamine: plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.
성분
Contains 1.0mg/ml recombinant human insulin, 0.55mg/ml human transferrin (substantially iron-free), 0.5μg/ml sodium selenite and 0.2mg/ml ethanolamine at the 100x concentration.
제조 메모
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Lixin Zhu et al.
American journal of physiology. Cell physiology, 295(1), C192-C202 (2008-05-16)
In a comparison of three different tissues, the membrane cytoskeleton linker protein ezrin was found to assume high levels of phosphorylation on threonine-567 (T567) in the brush border membranes of renal proximal tubule cells and small intestine enterocytes, in contrast
H Murakami et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(4), 1158-1162 (1982-02-01)
A serum-free medium supplemented with a few growth factors was devised to grow lymphocyte hybridomas. The medium was developed with the hybridoma line MPC11-BL, a fusion product between a mouse plasmacytoma cell line (MPC11TG70na3) and mouse (BALB/c) spleen cells. In
Lixin Zhu et al.
American journal of physiology. Cell physiology, 293(3), C874-C884 (2007-06-08)
In its dormant state, the membrane cytoskeletal linker protein ezrin takes on a NH(2) terminal-to-COOH terminal (N-C) binding conformation. In vitro evidence suggests that eliminating the N-C binding conformation by Thr(567) phosphorylation leads to ezrin activation. Here, we found for
Rawan Eid et al.
Biochimica et biophysica acta. Molecular cell research, 1864(2), 399-430 (2016-12-13)
Iron is an essential micronutrient that is problematic for biological systems since it is toxic as it generates free radicals by interconverting between ferrous (Fe2+) and ferric (Fe3+) forms. Additionally, even though iron is abundant, it is largely insoluble so
Nico Laur et al.
PloS one, 15(5), e0233357-e0233357 (2020-05-21)
Trace elements and minerals are compounds that are essential for the support of a variety of biological functions and play an important role in the formation of and the defense against oxidative stress. Here we describe a technique, allowing sequential
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