SAB5900202

Alpaca Anti-PD-L1, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• 동의어(들): B7-H, B7H1, PD-L1, PDCD1LG1, PDL1
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: alpaca
• Quality Level: 100
• 재조합: expressed in E. coli
• 결합: unconjugated
• 항체 형태: affinity purified VHH antibody
• 클론: PL03, monoclonal
• 분석: >95% (SDS-PAGE)
• 형태: lyophilized solid
• 분자량: 14.4 kDa; ~ 15-18 kDa (SDS PAGE under reducing condition)
• 정제법: immobilized metal affinity chromatography (IMAC)
• 종 반응성: human, (Cynomolgus macaques)
• 농도: 1 mg/mL
• 기술: ELISA: 1:10000-1:100000; flow cytometry: 1:50-1:100; immunofluorescence: 1:200-1:800; immunohistochemistry: 1:200-1:800; western blot: 1:1000-1:3000
• 색상: colorless
• pH: ( in PBS, pH 7.4)
• 동형: VHH
• 단백질 ID 수납 번호: Q9NZQ7.1
• UniProt 수납 번호: Q9NZQ7
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CD274(29126)

설명

일반 설명

PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis.Alpaca derived anti-PD-L1 VHH single domain antibody (molecular weight: 14.4kDa), expressed in E. coli under conditions free from animal derived components. It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. The small size and monovalency makes it a superior tool in microsopy. These single-domain antibodies offer superior chemical (wide variety of pH, urea, etc) and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

특이성

Human Programmed Death-Ligand 1 (PD-L1)

면역원

Recombinant human PD-L1 (Phe19-Tyr134) protein

애플리케이션

ELISA, WB, FC, IHC, IF

포장

50 µg per vial / 100 µg per vial

물리적 형태

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.5

재구성

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

저장 및 안정성

Stable for 1 year at 4°C from date of receipt.

기타 정보

Storage at 4°C; Do not freeze.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable