추천 제품
생물학적 소스
alpaca
Quality Level
재조합
expressed in E. coli
결합
unconjugated
항체 형태
affinity purified VHH antibody
클론
EB02, monoclonal
분석
>95% (SDS-PAGE)
형태
lyophilized solid
분자량
13.7 kDa
~15-18 kDa
정제법
immobilized metal affinity chromatography (IMAC)
종 반응성
human
농도
1 mg/mL
기술
immunofluorescence: 1:200-1:600
immunohistochemistry: 1:200-1:600
색상
colorless
pH
( in PBS, pH 7.4)
동형
VHH
단백질 ID 수납 번호
UniProt 수납 번호
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... ERBB2(2064)
일반 설명
Receptor tyrosine-protein kinase erbB-2, also known as Her2, is a protein that in humans is encoded by the ERBB2 gene. The ErbB family consists of four closely related tyrosine kinase receptors that act as potent mediators of normal cell growth and development. Aberrant expression or function of one or more of these receptors can play a major role in the development and evolution of cancer. Her2 has been implicated in the evolution of both breast and gastric cancers, and is evident in other cancer types such as ovarian and salivary gland tumors. Her2 possesses an active tyrosine kinase domain, but no direct ligand has been identified yet. Her2 is the preferred binding partner to the other members of the ErbB family and is thought to act primarily through the Ras-MAPK, PI3k-PKB/Akt, and PLC-PKC signalling pathways.Alpaca derived anti-HER2 VHH single domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components. It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
특이성
Human Epidermal Growth Factor Receptor 2 (Her2)
면역원
Recombinant human HER2 (Thr23-Thr652) protein
애플리케이션
IHC,IF
포장
50 µg per vial / 100 µg per vial
물리적 형태
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.8
재구성
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
저장 및 안정성
Stable for 1 year at 4°C from date of receipt.
기타 정보
Storage at 4°C; Do not freeze.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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