SRE0047

Proteinase K from Tritirachium album

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥30 units/mg protein, for molecular biology

• 동의어(들): Endopeptidase K
• CAS Number: 39450-01-6
• 효소 위원회 번호: 3.4.21.64 (BRENDA, IUBMB)
• EC Number: 254-457-8
• MDL number: MFCD00132129
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: fungus (Tritirachium album)
• Quality Level: 400
• Grade: for molecular biology
• 형태: lyophilized powder
• 특이 활성도: ≥30 units/mg protein
• 분자량: 28.93 kDa
• 불순물: ≤1 ppm DNA
• 응용 분야: diagnostic assay manufacturing
• 외래 활성: DNAse, Nickase and RNAse, none detected
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Proteinase K is an extracellular endopeptidase. It is synthesized by the mold Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein with a molecular weight of 28,930 Da. Active proteinase K is characterized with an unhydrolysed protein chain and autolysed polypeptide chains.

애플리케이션

Proteinase K catalyzes the hydrolysis of keratin.

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

생화학적/생리학적 작용

Proteinase K catalyzes the hydrolysis of keratin.

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

단위 정의

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable