SRP0108

HDAC-6 human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• 동의어(들): HD6, Histone deacetylase 6, JM21
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected insect cells
• 분석: ≥80% (SDS-PAGE)
• 형태: aqueous solution
• 분자량: 159 kDa
• 포장: pkg of 50 μg
• NCBI 수납 번호: BC069243
• UniProt 수납 번호: Q9UBN7
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... HDAC6(10013)

설명

일반 설명

HDAC6 (histone deacetylase 6) is a member of the HDAC family of enzyme which are generally linked with gene expression and chromatin dynamics. This protein resides exclusively in the cytoplasm and is present in the perinuclear punctate structures as well as the leading edges of the cell.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

생화학적/생리학적 작용

HDAC6 (histone deacetylase 6) functions as a microtubule or tubulin deacetylase, which in turn regulates microtubule-dependent chemotactic cell motility. This protein shows colocalization with p150^glued protein, a dynactin component. It contains a ubiquitin binding zinc finger, and interacts with ubiquitinated proteins post-proteasomal inhibition. It is essential for the survival of inflammatory breast cancers (IBCs), though does not function as an oncogene. Treatment of IBC cells with HDAC6 inhibitor ricolinostat (ACY1215) results in the inhibition of IBC cell proliferation both in vitro and in vivo.

단위 정의

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

물리적 형태

Formulated in 40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween20, 3mM DTT, and 20% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable