추천 제품
생물학적 소스
human
재조합
expressed in baculovirus infected insect cells
분석
≥70% (SDS-PAGE)
양식
aqueous solution
분자량
49 kDa (LKB1)
66 kDa (MO25)
74 kDa (STRAD)
포장
pkg of 20 μg
저장 조건
avoid repeated freeze/thaw cycles
농도
>0.02 mg/mL
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−70°C
유전자 정보
human ... STK11(6794)
일반 설명
The product is a complex of human Liver kinase B1 (LKB1), also known as serine/threonine kinase 11 (STK11) (GenBank Accession No. NM_000455; amino acids 2-433, with N-terminal His-tag), mouse protein- 25 (MO25) or calcium binding protein 39 (CAB39) (GenBank Accession No. NM_016289; amino acids 2-341, with N-terminal GST-tag) and STE20-related kinase adaptor alpha (STRAD) (GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag).
애플리케이션
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
생화학적/생리학적 작용
Liver kinase B1 (LKB1), also known as serine/threonine kinase 11 (STK11), is a serine/threonine kinase that regulates cell polarity and functions as a tumor suppressor protein. LKB1 regulates the activity of adenosine monophosphate-activated protein kinase (AMPK), promoting the suppression of growth and proliferation under energetically unfavorable conditions. Mouse protein-25 (MO25) or calcium binding protein 39 (CAB39) is a scaffolding protein which modulates the activities of STE20 family protein kinases. The MO25a isoform is part of the LKB1 tumor suppressor complex. STE20-related kinase adaptor α (STRAD) is a pseudokinase. It forms a heterotrimeric complex with LKB1 and MO25. The LKB1/MO25/STRAD complex modulates the metabolism, proliferation and cellular polarity of human intestinal epithelial cells.
물리적 형태
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
제조 메모
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Structural insights into the activation of MST3 by MO25.
Mehellou Y
Biochemical and Biophysical Research Communications, 431(3), 604-609 (2013)
Naomi X Y Ling et al.
Nature metabolism, 2(1), 41-49 (2020-01-30)
Central to cellular metabolism and cell proliferation are highly conserved signalling pathways controlled by mammalian target of rapamycin (mTOR) and AMP-activated protein kinase (AMPK)1,2, dysregulation of which are implicated in pathogenesis of major human diseases such as cancer and type
P A Marignani et al.
Cancer biology & therapy, 6(10), 1627-1631 (2007-10-09)
STRADalpha is a pseudokinase that forms a heterotrimeric complex with the scaffolding protein MO25 and the tumor suppressor serine threonine protein kinase LKB1. Mutations in the LKB1 gene are responsible for the Peutz-Jeghers Syndrome (PJS) characterized by a predisposition to
Yue Zong et al.
Cell research, 29(6), 460-473 (2019-04-06)
AMPK, a master regulator of metabolic homeostasis, is activated by both AMP-dependent and AMP-independent mechanisms. The conditions under which these different mechanisms operate, and their biological implications are unclear. Here, we show that, depending on the degree of elevation of
Kuo-Hui Su et al.
Molecular cell, 76(4), 546-561 (2019-09-29)
Through transcriptional control of the evolutionarily conserved heat shock, or proteotoxic stress, response, heat shock factor 1 (HSF1) preserves proteomic stability. Here, we show that HSF1, a physiological substrate for AMP-activated protein kinase (AMPK), constitutively suppresses this central metabolic sensor.
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