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Merck
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Key Documents

T1299

Sigma-Aldrich

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse

clone TH-2, ascites fluid

동의어(들):

Anti-Tyrosine Hydroxylase, Mouse Anti-Tyrosine Hydroxylase, Tyrosine Hydroxylase Detection

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

TH-2, monoclonal

포함

15 mM sodium azide

종 반응성

human, monkey, guinea pig, rat, sheep, bovine, rabbit

기술

immunohistochemistry: suitable
immunoprecipitation (IP): suitable using native or SDS-denatured TH.
western blot: 1:10,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.

동형

IgG1

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... TH(7054)
rat ... Th(25085)

일반 설명

Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tyrosine hydroxylase (TH) RNA undergoes alternative splicing resulting in four isoforms of TH subunit protein in humans. The four human isoforms are TH-1 (55.6 kDa), TH-2 (56 kDa), TH-3 (58.1 kDa), and TH-4 (58.5 kDa). Only humans express all four isoforms.
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor.

특이성

The antibody recognizes an epitope present in the N-terminal region (approx. aa 9-16) of both rodent and human tyrosine hydroxylase. Clone TH-2 reacts with the intact TH subunits.

면역원

rat tyrosine hydroxylase (TH).

애플리케이션

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in
  • western blotting
  • indirect immunofluorescence
  • immunostaining
  • immunohistochemistry
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunoprecipitation

Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in western blotting and immunocytochemistry.

생화학적/생리학적 작용

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Chemical sympathectomy-induced changes in TH-, VIP-, and CGRP-immunoreactive fibers in the rat mandible periosteum: Influence on bone resorption
Cherruau M, et al.
Journal of Cellular Physiology, 194(3), 341-348 (2003)
Essential role of mesolimbic brain-derived neurotrophic factor in chronic social stress-induced depressive behaviors
Koo JW, et al.
Biological Psychiatry, 80(6), 469-478 (2016)
Induction of neurite outgrowth in PC12 cells by the bacterial nucleoside N6-methyldeoxyadenosine is mediated through adenosine A2a receptors and via cAMP and MAPK signaling pathways
Charles MP, et al.
Biochemical and biophysical research communications, 304(4), 795-800 (2003)
Multiple genes on chromosome 7 regulate dopaminergic amacrine cell number in the mouse retina
Whitney IE, et al.
Investigative Ophthalmology & Visual Science, 50(5), 1996-2003 (2009)
Human tyrosine hydroxylase isoforms inhibition by excess tetrahydropterin and unusual behavior of isoform 3 after cAMP-dependent protein kinase phosphorylation
Alterio J, et al.
Test, 273(17), 10196-10201 (1998)

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