T3069
Trizma® hydrochloride solution
pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture
동의어(들):
Tris hydrochloride solution
제품 이름
Trizma® hydrochloride solution, pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture
grade
BioPerformance Certified
Molecular Biology
form
solution
concentration
2 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)
Quality Level
color
colorless
pH
7.95—8.05
8.0
useful pH range
7.0-9.0
density
1.07—1.09 g/cm3
absorption
≤0.05 at 290 at 40%
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Application
Trizma® hydrochloride solution has been used as a component of lysis buffer for testing on commercially available biological sample preservation paper types. It has also been used as a vehicle and for dissolving leptin in reporter gene assay studies.
General description
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Tris(hydroxymethyl)aminomethane (Tris) has a pH buffering range between 7.0 - 9.0 and is a routine buffer in biological sciences. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. It displays negligible binding towards metal in solutions.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
A review of acellular immersion tests on bioactive glasses-influence of medium on ion release and apatite formation
Nommeots-Nomm A, et al.
International Journal of Applied Glass Science, 11(3) (11)
(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39) (5)
Universal buffers for use in biochemistry and biophysical experiments
Brooke D, et al.
AIMS Biophysics, 2(3) (2)
Optimal DNA extractions from blood on preservation paper limits conservation genomic but not conservation genetic applications
Stowell SML, et al.
Journal for nature conservation, 46 (46)
Elke Ericson et al.
Molecular and cellular endocrinology, 412, 257-264 (2015-05-20)
In a world with increasing incidences of obesity, it becomes critical to understand the detailed regulation of appetite. To identify novel regulators of the signaling mediated by one of the key hormones of energy homeostasis, leptin, we screened a set
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