추천 제품
제품명
Monoclonal Anti-α-Tubulin antibody produced in mouse, ascites fluid, clone B-5-1-2
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
B-5-1-2, monoclonal
분자량
antigen ~50 kDa
포함
15 mM sodium azide
종 반응성
mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat
향상된 검증
independent ( Antibodies)
Learn more about Antibody Enhanced Validation
기술
indirect immunofluorescence: 1:2,000 using cultured human or chicken fibroblasts
radioimmunoassay: suitable
western blot: 1:4,000 using human fibroblast cell extract
동형
IgG1
UniProt 수납 번호
응용 분야
research pathology
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
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일반 설명
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the B-5-1-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin is the major building block of microtubules.
The TUBA4A (tubulin α 4a), is mapped to human chromosome 2q35. The gene codes for a member of the a -tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. The α-subunit of tubulin has molecular weight of 50,000.
특이성
Monoclonal Anti-alpha-Tubulin antibody recognizes an epitope at the C-terminal end of the α-tubulin isoform in various organisms.
Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.
면역원
Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
애플리케이션
Monoclonal Anti-α-Tubulin antibody produced in mouse has been used:
- In immunofluorescence Analysis
- In western blotting/ Immunoblotting
- For immunolabelling cells in electron microscopy
생화학적/생리학적 작용
Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS).
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton.
α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS).
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton.
Tubulin α mediates the formation of cytoskeletal protein polymers. It is necessary for cell cycle and controls cellular movement and signaling. Tubulin α is known to maintain the shape of the cells. It is an active part of oncogenesis and progression of different types of cancer. Posttranslationally modified Tubulin α governs microtubule functions including cell trafficking and cellular tensegrity.
물리적 형태
Liquid
저장 및 안정성
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Zhixiong Dong et al.
International journal of medical sciences, 16(8), 1132-1141 (2019-09-17)
Ribosomal biogenesis is responsible for protein synthesis in all eukaryotic cells. Perturbation of ribosomal biogenesis processes can cause dysfunctions of protein synthesis and varieties of human diseases. In this study, we examine the role of RPL15, a large ribosomal subunit
TUBA4A gene analysis in sporadic amyotrophic lateral sclerosis: identification of novel mutations.
Pensato V, et al.
Journal of Neurology, 262(5), 1376-1378 (2015)
Increased expression of αTubulin is associated with poor prognosis in patients with pancreatic cancer after surgical resection
Lin C, et al.
Oncotarget, 7(37), 60657-60657 (2016)
Florian Sonthonnax et al.
Scientific reports, 9(1), 12171-12171 (2019-08-23)
Phosphoprotein (P) and matrix protein (M) cooperate to undermine the immune response to rabies virus (RABV) infections. While P is involved in the modulation of the Jak-Stat pathway through the cytoplasmic retention of interferon (IFN)-activated STAT1 (pSTAT1), M interacts with
Centrosomes split in the presence of impaired DNA integrity during mitosis.
Hut HM
Molecular and Cellular Biology, 14(5), 1993-2004 (2003)
문서
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
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