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Merck
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문서

TRUE6

Sigma-Aldrich

TrueGel3D Hydrogel Kits

SLO-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker

동의어(들):

3D Hydrogel Kit, TrueGel3D Kit

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

배송 상태

dry ice

저장 온도

−70°C

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관련 카테고리

일반 설명

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-DEXTRAN does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

애플리케이션

TRUE6-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic natural tissue stiffness and to investigate the effect of mechanical stimulus on cell physiology and behavior. It is also used to culture non-proliferating cells and proliferating spheroids that do not attach to hydrogel for survival, but rather use their own extracellular matrix.

특징 및 장점

  • Slow gelling (takes 8 minutes to 25 minutes, depends on gel stiffness)
  • Flexibility in modulating gel stiffness
  • Does not allow cell migration
  • Allow cell recovery and post-culture analyses
  • Cell inert hydrogel (if not customized with RGD peptide)

성분

TRUE6-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

주의사항

Avoid prolong exposure of PEG non cell-degradable crosslinker to air.

픽토그램

Corrosion
GHS05

신호어

Warning

유해 및 위험 성명서

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

프로토콜

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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