V7259

Vascular Endothelial Growth Factor human

≥98% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

• 동의어(들): VEGF
• CAS Number: 127464-60-2
• MDL number: MFCD00286542
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 제품명: Vascular Endothelial Growth Factor human, VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥98% (SDS-PAGE)
• 양식: powder
• 효능: 1-10 ng/mL ED₅₀/EC₅₀
• 품질: endotoxin tested
• 분자량: protein 38.2 kDa (as homodimer)
• 포장: pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤0.1 EU/μg
• 색상: white
• solubility: water: soluble
• UniProt 수납 번호: P15692
• 저장 온도: −20°C
• 유전자 정보: human ... VEGFA(7422)

설명

일반 설명

Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.

애플리케이션

Vascular Endothelial Growth Factor (VEGF) has been used for-

• the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival
• inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice

생화학적/생리학적 작용

Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.

VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.

물리적 특성

Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa

물리적 형태

Lyophilized from a sterile filtered buffered solution with no additives.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)