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Merck
모든 사진(2)

주요 문서

W1754

Sigma-Aldrich

Water

PCR Reagent, suitable for PCR

동의어(들):

H2O

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About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
UNSPSC 코드:
12191602
PubChem Substance ID:
NACRES:
NA.25
Grade:
PCR Reagent
기술:
PCR: suitable
bp:
100 °C (lit.)
vapor pressure:
3 mmHg

제품명

Water, PCR Reagent

Grade

PCR Reagent

Quality Level

vapor density

<1 (vs air)

vapor pressure

3 mmHg

무균

sterile-filtered

양식

liquid

포장

vial of 1.5 mL

기술

PCR: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

외래 활성

DNase, none detected
RNase, none detected

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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일반 설명

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

애플리케이션

Water has been used:

  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
  • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

적합성

Suitable for polymerase chain reaction (PCR)

기타 정보

Easily compare specifications for Water products with the Water specification table.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves


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문서

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

프로토콜

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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