Z0627

Monoclonal Anti-ZAP-70 antibody produced in mouse

~1.5 mg/mL, clone 1E7.2, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD00803577
• UNSPSC 코드: 12352203
• NACRES: NA.44

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 1E7.2, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~70 kDa
• 종 반응성: human, mouse
• 포장: antibody small pack of 25 μL
• 농도: ~1.5 mg/mL
• 기술: flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.5-1.0 μg/mL using Jurkat cell extract
• 동형: IgG1
• UniProt 수납 번호: P43403
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ZAP70(7535) ; mouse ... Zap70(22637)

설명

일반 설명

Monoclonal Anti-ZAP-70 (mouse IgG1 isotype) is derived from the hybridoma 1E7.2 produced by the fusion of mouse myeloma cells and splenocytes from mice. ZAP-70 is a protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells.

면역원

The immunogen sequence is conjugated to KLH.

peptide corresponding to amino acids 282-307 of human ZAP-70.

애플리케이션

Monoclonal Anti-ZAP-70 antibody has been used in

• enzyme linked immunosorbent assay (ELISA)
• immunoblottin
• flow cytometry
• immunoprecipitation
• immunocytochemistry

Monoclonal Anti-ZAP-70 antibody is suitable for use in western blot (0.5-1.0 μg/mL using Jurkat cell extract), flow cytometry, immunocytochemistry, immunoprecipitation, microarray and indirect ELISA.

생화학적/생리학적 작용

ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the zeta chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling . Lack of ZAP-70 leads to an absence of antigen-specific response and also causes impaired T cell differentiation . Anti-ZAP-70 antibody is specific for human and mouse ZAP-70 (approx. 70 kDa).

Zeta-chain-associated protein kinase70 (ZAP-70) is required for T-cell activation and is activated by(Tyrosine protein kinase) Lck-mediated phosphorylation of its tyrosine residues. Mutation of the gene that creates a defective ZAP-70 results in severe combined immunodeficiency syndrome (SCID).

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)