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Merck
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주요 문서

ZRB1355

Sigma-Aldrich

Anti-Cardiac Troponin I Antibody, clone 1F23, ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

동의어(들):

Troponin I

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit (recombinant)

Quality Level

재조합

expressed in HEK 293 cells

결합

unconjugated

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

1F23, monoclonal
recombinant monoclonal

제품 라인

ZooMAb® learn more

형태

lyophilized

분자량

calculated mol wt 24.0 kDa
observed mol wt ~26 kDa

종 반응성

human, rat, mouse

포장

antibody small pack of 25 μL

환경친화적 대안 제품 특성

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

향상된 검증

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

기술

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG

UniProt 수납 번호

환경친화적 대안 카테고리

배송 상태

ambient

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... TNNI1(7135)

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

특이성

Clone 1F23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects cardiac troponin I. It targets an epitope within 14 amino acids from the C-terminal region.

면역원

KLH-conjugated liner peptide corresponding to 14 amino acids from the C-terminal region of human Cardiac Troponin I.

애플리케이션

Anti-Cardiac Troponin I, clone 1F23 ZooMAb, Cat. No. ZRB1355, a Rabbit monoclonal antibody that targets Cardiac Troponin I and is tested in Immunocytochemisty, Immunofluorescence, Immunohistochemistry and Western Blotting.
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cardiac Troponin I in human heart tissue lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cardiac Troponin I in AC16 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cardiac Troponin I in human heart and rat heart tissue sections.

Immunofluorescence Analysis: A 1:1000 dilution from a representative lot detected Cardiac Troponin I in human heart muscle tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Troponin I, cardiac muscle (UniProt: P19429; also known as Cardiac troponin I) is encoded by the TNNI3 (also known as TNNC1) gene (Gene ID: 7137) in human. Troponin is a complex that regulates the contraction of skeletal muscle. It consists of 3 subunits known as C, T, and I, which are located periodically along the thin filament of the myofibrils. Troponins are found in skeletal and cardiac muscle, but not in smooth muscle. Troponin C (TnC) binds calcium ions, Troponin I is the inhibitory subunit of troponin that inhibits actomyosin ATPase and Troponin T binds to tropomyosin. It also confers calcium-sensitivity to actomyosin ATPase. Amino acids region 32-79 of Troponin I is involved in binding to TnC and region 129-149 is involved in binding to both TnC and actin. Cardiac muscle Troponin I has 31 amino acid residues at its N-terminal that are not present in the skeletal muscle form. Hence, cardiac specificity of this isoform is shown to offer accuracy in diagnosis in acute or chronic skeletal muscle injury. Mutation in TNNI3 gene are reported to result in multiple types of cardiomyopathies that can result in premature death. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, H-C., and Fajer, PG (1998). Biochemistry 37(19); 6628-6635; Skeik, N., and Patel, DC (2007). Int. J. Angiol. 16(2); 53-58).

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

재구성

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Jara M Baena-Montes et al.
Journal of cardiovascular development and disease, 9(8) (2022-08-26)
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