추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
1F4, recombinant monoclonal
설명
1F4 Clone
제품 라인
ZooMAb® learn more
양식
lyophilized
분자량
calculated mol wt 48.46 kDa
observed mol wt ~45 kDa
정제법
using Protein A
종 반응성
rat, human, mouse
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG
에피토프 서열
N-terminal half
단백질 ID 수납 번호
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... GATM(2628)
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
특이성
Clone 1F4 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Glycine amidinotransferase, mitochondria (GATM/AGAT). It targets an epitope within 22 amino acids from the N-terminal half.
면역원
KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal half of human Glycine amidinotransferase, mitochondrial (GATM/AGAT).
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in Rat kidney tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GATM/AGAT in Rat Kidney tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected GATM/AGAT in Human Kidney and Mouse Kidney tissue lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GATM/AGAT in Human umbilical vein endothelial cells (HUVEC).
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected GATM/AGAT in human kidney tissues sections.
Affinity Binding Assay: A representative lot of this antibody bound GATM/AGAT peptide with a KD of 1.2 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GATM/AGAT in human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Rat kidney tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GATM/AGAT in Rat Kidney tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected GATM/AGAT in Human Kidney and Mouse Kidney tissue lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GATM/AGAT in Human umbilical vein endothelial cells (HUVEC).
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected GATM/AGAT in human kidney tissues sections.
Affinity Binding Assay: A representative lot of this antibody bound GATM/AGAT peptide with a KD of 1.2 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GATM/AGAT in human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
표적 설명
Glycine amidinotransferase, mitochondrial (UniProt: P50440; also known as EC:2.1.4.1, L-arginine:glycine amidinotransferase, Transamidinase) is encoded by the GATM (also known as AGAT) gene (Gene ID: 2628) in human. GATM is a homodimeric protein that catalyzes the rate limiting step in the biosynthesis of guanidinoacetate, the immediate precursor of creatine. It displays an equilibrium between the monomeric and dimeric forms. It is synthesized with a mitochondrial transit peptide (aa 1-37), which is subsequently cleaved off to produce the active enzyme (aa 38-423). Its expression is observed in various tissues, with the highest expression observed in kidney. Its expression is elevated in the myocardium during cardiac failure. It may play a role in embryonic and central nervous system development and may also be involved in response to cardiac failure by elevating local creatine synthesis. Two isoforms of GATM have been described. Isoform 1 is localized in the mitochondrial inner membrane and isoform 2 is cytoplasmic. Mutations in GATM gene have been linked to cerebral creatine deficiency syndrome 3, an autosomal recessive disorder that is characterized by developmental delay, intellectual disability, and myopathy characterized by muscle weakness and atrophy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cullen, ME., et al. (2006). Circulation. 114(1 suppl); 116-120; Gross, MD., et al. (1986). Arch. Biochem. Biophys. 251(2); 747-755).
물리적 형태
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
재구성
0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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