추천 제품
product name
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Agency
suitable for USP L43
제품 라인
Discovery®
특징
endcapped
제조업체/상표
Discovery®
포장
1 ea of
라벨링 범위
12% Carbon loading
파라미터
≤70 °C temp. range
400 bar pressure (5801 psi)
기술
HPLC: suitable
LC/MS: suitable
길이 × I.D.
15 cm × 4.6 mm
표면적
300 m2/g
표면 범위
4 μmol/m2
불순물
<10 ppm metals
기질
silica gel, high purity, spherical particle platform
fully porous particle
기질 활성군
PFP (pentafluorophenyl) phase
입자 크기
3 μm
공극 크기
120 Å
operating pH range
2-8
응용 분야
food and beverages
분리 기술
reversed phase
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일반 설명
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
애플리케이션
- Nature of the main contaminant in the drug primaquine diphosphate: SFC and SFC-MS methods of analysis.: This study uses the Discovery® HS F5 HPLC Column to identify and quantify the main contaminant in primaquine diphosphate, crucial for ensuring the quality and safety of this antimalarial drug (Brondz et al., 2007).
- HPLC assisted chemobiological standardization of alpha-glucosidase-I enzyme inhibitory constituents from Piper longum Linn-An Indian medicinal plant.: Demonstrates the application of the Discovery® HS F5 HPLC Column in the biologically relevant chemical analysis of Indian medicinal plants, contributing to the standardization of herbal medicine (Pullela et al., 2006).
- Disposition study of a new potential antineoplastic agent dimefluron in rats using high-performance liquid chromatography with ultraviolet and mass spectrometric detection.: Utilizes the Discovery® HS F5 HPLC Column to study the pharmacokinetics and metabolism of a novel anticancer agent, aiding in the development of effective cancer therapies (Císar et al., 2005).
- A simplified analytical method for a phenotyping cocktail of major CYP450 biotransformation routes.: This research employs the Discovery® HS F5 HPLC Column to simplify the analysis of a phenotyping cocktail, enhancing the assessment of drug metabolism and facilitating personalized medicine approaches (Jerdi et al., 2004).
특징 및 장점
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
법적 정보
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.
Koji Baba et al.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
Umberto M Battisti et al.
Journal of chromatography. A, 1443, 152-161 (2016-03-30)
A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.
Chromatograms
application for SPE, application for HPLC자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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