추천 제품
product name
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L43
제품 라인
Discovery®
특징
endcapped
제조업체/상표
Discovery®
포장
1 ea of
라벨링 범위
12% Carbon loading
파라미터
≤70 °C temp. range
400 bar pressure (5801 psi)
기술
HPLC: suitable
LC/MS: suitable
길이 × I.D.
15 cm × 4 mm
표면적
300 m2/g
표면 범위
4 μmol/m2
불순물
<10 ppm metals
기질
silica gel, high purity, spherical particle platform
fully porous particle
기질 활성군
PFP (pentafluorophenyl) phase
입자 크기
5 μm
공극 크기
120 Å
operating pH range
2-8
응용 분야
food and beverages
분리 기술
reversed phase
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일반 설명
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
특징 및 장점
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
법적 정보
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Se pu = Chinese journal of chromatography, 27(2), 216-219 (2009-07-25)
A simple method was developed for the analysis of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline (6-OH-MTHbetaC), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) in rat brain by high performance liquid chromatography with electrochemical detection (HPLC-ECD). The separation of the sample was performed on a Discovery
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