Documents
D9542
DAPI
for nucleic acid staining
Recommended Products
grade
for molecular biology
Quality Level
Assay
≥98% (HPLC)
form
powder
technique(s)
transfection: suitable
solubility
H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
PBS: insoluble
ε (extinction coefficient)
30 at 263 nm in H2O at 1 mM
fluorescence
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability
suitable for fluorescence
storage temp.
2-8°C
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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Related Categories
1 of 4
This Item | MBD0015 | MBD0020 | D8417 |
|---|---|---|---|
| assay ≥98% (HPLC) | assay - | assay - | assay ≥98% (HPLC and TLC) |
| form powder | form liquid | form liquid | form powder |
| technique(s) transfection: suitable | technique(s) transfection: suitable | technique(s) DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable | technique(s) cell culture | mammalian: suitable |
| solubility H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.), PBS: insoluble | solubility - | solubility - | solubility H2O: 20 mg/mL (Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.), PBS: insoluble |
| ε (extinction coefficient) 30 at 263 nm in H2O at 1 mM | ε (extinction coefficient) - | ε (extinction coefficient) - | ε (extinction coefficient) 30 at 263 nm in H2O |
General description
Application
- DNA staining in agarose gels
- analysis of changes in DNA during apoptosis
- detection of mycoplasma
- photofootprinting of DNA
- immunofluorescent staining of cells
DAPI has been used:-
- in rapid monitoring of microbial contamination
- in chromosomal banding technique
- in detection of apoptotic cells
- in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
- to stain mature pollen grains(0.5 mg/ml)
Biochem/physiol Actions
Caution
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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