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F6057

Sigma-Aldrich

Fluoroshield with DAPI

histology mounting medium

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NACRES:
NA.47

Quality Level

form

liquid

pH range

7.9-8.3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

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This Item
D9564F6182F6937
DAPI, dilactate ≥98% (HPLC)

Sigma-Aldrich

D9564

DAPI, dilactate

Sigma-Aldrich

Sigma-Aldrich

F6182

Fluoroshield

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

100

pH range

7.9-8.3

pH range

-

pH range

7.8-8.2

pH range

8.0-8.4

General description

Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Application

Fluoroshield with DAPI has been used:
  • as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
  • as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
  • as mounting solution for immunostained heart sections for nuclei detection

Legal Information

FluoroShield is a trademark of ImmunoBioScience Corp.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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