Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

View All Documentation

P5542

Sigma-Aldrich

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

lyophilized powder, ≥1,000 units/mg protein (Lowry)

Synonym(s):

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

Sign Into View Organizational & Contract Pricing

Select a Size

5 UNITS
$285.00
25 UNITS
$972.00

$285.00

In StockDetails

Request a Bulk Order
All Photos(3)

Select a Size

Change View
5 UNITS
$285.00
25 UNITS
$972.00

About This Item

CAS Number:
37288-19-0
Enzyme Commission number:
4.6.1.13 (BRENDA, IUBMB)
EC Number:
232-638-2
MDL number:
MFCD00131896
UNSPSC Code:
12352204
NACRES:
NA.32

$285.00

In StockDetails

Request a Bulk Order

form

lyophilized powder

Quality Level

300

specific activity

≥1,000 units/mg protein (Lowry)

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein

storage temp.

−20°C

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
P8804525186P6621
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus lyophilized powder, ≥1,000 units/mg protein (Lowry)

P5542

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

P8804

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

Phospholipase C, Bacillus cereus

525186

Phospholipase C, Bacillus cereus

Phospholipase C from Bacillus cereus ≥200 units/mg protein

P6621

Phospholipase C from Bacillus cereus

form

lyophilized powder

form

buffered aqueous glycerol solution

form

solid

form

lyophilized powder

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

300

specific activity

≥1,000 units/mg protein (Lowry)

specific activity

≥1,000 units/mg protein (Lowry)

specific activity

≥50 units/mg dry wt.

specific activity

≥200 units/mg protein

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein

foreign activity

-

foreign activity

-

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

Gene Information

-

Gene Information

Bacillus cereus ATCC 10987 ... plC(2749087)

General description

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.[1]

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane.[2][3] It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.[4]

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Unit Definition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Physical form

Contains phosphate buffer salts, EDTA and stabilizer

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

inhibitor

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000419119
    0000419119
    0000411483
    0000411483
    0000386550

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Helen Neuert et al.
    Development (Cambridge, England), 147(2) (2019-12-22)
    The development of tissues and organs requires close interaction of cells. To achieve this, cells express adhesion proteins such as the neural cell adhesion molecule (NCAM) or its Drosophila ortholog Fasciclin 2 (Fas2). Both are members of the Ig-domain superfamily
    Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.
    Brameshuber M
    The Journal of Biological Chemistry, 285(53), 41765-41771 (2010)
    Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol.
    Heinz DW
    The Embo Journal, 14(16), 3855-3863 (1995)
    I Shakuntala et al.
    Veterinary microbiology, 117(2-4), 229-234 (2006-07-25)
    Listeria monocytogenes, a gram-positive, facultative intracellular pathogen was isolated from buffaloes with a history of reproductive disorders and polymerase chain reaction (PCR) analyses for the presence of virulence-associated genes were conducted. A total of 530 samples of faecal, nasal, vaginal
    Xia Liu et al.
    Endothelium : journal of endothelial cell research, 13(3), 205-211 (2006-07-15)
    In the authors' previous studies, they found that phosphatidylcholine-specific phospholipase C (PC-PLC) and phosphatidylinositol-specific phospholipase C (PI-PLC) played contrary roles in the apoptosis of vascular endothelial cells (VECs), but the mechanism underlying the phenomenon remains unclear. To address this question
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service