X114

Triton^™ X-114

laboratory grade

• Synonym(s): (1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: (C₂H₄O)n C₁₄H₂₂O, n = 7 or 8
• CAS Number: 9036-19-5
• Beilstein/REAXYS Number: 2315025
• MDL number: MFCD00128254
• UNSPSC Code: 12161900
• PubChem Substance ID: 24902129
• NACRES: NA.21

Properties

• grade: laboratory grade
• Quality Level: 200
• description: non-ionic
• mol wt: average mol wt 537
• composition: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x = 7-8
• refractive index: n20/D 1.488 (lit.)
• CMC: 0.2 mM (20-25°C)
• transition temp: pour point ~−9 °C; cloud point 23 °C
• density: 1.058 g/mL at 25 °C (lit.)
• HLB: 12.4

Description

General description

Triton^™ X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins.[1] Solution of Triton^™ X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C.[2] Phase separation of proteins in solutions of Triton^™ X-114 has been investigated.[1] This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton^™ X-114 has been used:

• To remove endotoxin from solutions of protein using phase separation technique[3]
• In the extraction and purification of DNA from endotoxin[4]
• In the biphasic extraction of lipoproteins from bacterial cell wall[5][6]
• As a non-ionic detergent with a low cloud point (23 °C) enabling protein solubilization with phase-partitioning of hydrophilic from amphiphilic proteins

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• pictograms: GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H315,H318,H410
• pcodes: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: 483.8 °F - closed cup
• flash_point_c: 251 °C - closed cup
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter