93443

Triton^™ X-100 solution

BioUltra, for molecular biology, ~10% in H₂O

• Synonym(s): Triton^™ X-100, 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• CAS Number: 9036-19-5
• Beilstein: 2315025
• MDL number: MFCD00128254
• PubChem Substance ID: 57653560

Properties

• grade: for molecular biology
• Quality Level: 200
• description: non-ionic
• product line: BioUltra
• form: liquid
• mol wt: micellar avg mol wt 80,000; average mol wt 625
• concentration: ~10% in H₂O
• aggregation number: 100-155
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.349±0.002
• pH: 6.5-8.5 (25 °C)
• CMC: 0.2-0.9 mM (20-25°C); 0.23 mM
• transition temp: cloud point 65 °C; pour point ~7 °C
• density: 1.01 g/mL at 20 °C
• cation traces: Al: ≤1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤100 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤100 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• absorption: cut-off at 310 nm
• HLB: 13.5
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton-X has been used:

• for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
• As a component of lysis buffer used for homogenization of cells
• As a component of blocking buffer in immunofluorescence staining
• For the preparation of cells in double fluorescence staining

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Pictograms: GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H315 - H318 - H410
• Precautionary Statements: P273 - P280 - P302 + P352 - P305 + P351 + P338 + P310
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(C): 251 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves