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282103

Sigma-Aldrich

Triton X-100 reduced

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Synonym(s):
Polyoxyethylene (10) isooctylcyclohexyl ether
Linear Formula:
4-(C8H17)C6H10(OCH2CH2)nOH, n~10
CAS Number:
MDL number:
NACRES:
NA.23

Quality Level

refractive index

n20/D 1.473 (lit.)

density

1.029 g/mL at 25 °C (lit.)

InChI

1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3

InChI key

QQJNBKDKLMCALZ-UHFFFAOYSA-N

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This Item
X100PC93443303127
Triton™ X-100 reduced

Sigma-Aldrich

282103

Triton X-100 reduced

-
Triton™ X-100 peroxide- and carbonyl-free

Sigma-Aldrich

X100PC

Triton X-100

-
Triton™ X-100 solution BioUltra, for molecular biology, ~10% in H2O

Sigma-Aldrich

93443

Triton X-100 solution

Premium Grade
density

1.029 g/mL at 25 °C (lit.)

density

-

density

1.01 g/mL at 20 °C, 1.070 g/cm3

density

-

General description

Triton X-100 reduced (RTX-100) is formed when the benzene moiety of TX-100 is fully hydrogenated to a cyclohexane derivative. RTX-100 might enhance enzyme digestion and is also known to exhibit some effects on the photoisomerization of bacteriorhodopsin.
Triton X-100 reduced is a versatile nonionic surfactant with a hydrophilic polyethylene oxide chain, widely utilized as a laboratory detergent. Its applications span various areas, including cell biology and biochemical research. This detergent is commonly employed for tasks such as cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a crucial component in lysis buffers. Triton X-100 plays a key role in the isolation of lipid rafts, contributing to enhanced solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, Triton X-100 exhibits excellent wetting properties and facilitates emulsification.

In biochemical and cell biology research, Triton X-100 is instrumental in solubilizing membrane-bound proteins and isolating lipid rafts. Its unique properties allow for the preservation of the native conformation of proteins obtained from cellular membranes in solution. Triton X-100 reduced is derived from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative. This modified version, RTX-100, has demonstrated potential in enhancing enzyme digestion and influencing the photoisomerization of bacteriorhodopsin, showcasing its versatility and utility in advanced research applications.

Application

Triton X-100 reduced has been used:
  • as a component of LB-TT for the extraction of total protein from rat brains
  • in ADP-Glo assay and Cytophos adenosine triphosphatase (ATPase) assay
  • in phosphate-buffered saline (PBS) solution for the permeabilization of fibroblasts in 5′ ethynyl uridine staining, immunofluorescence, and immunolabeling

Features and Benefits

  • Non-ionic surfactant
  • Reduced polyoxyethylene content (~10)
  • Improves solubility and dispersibility of substances
  • Excellent wetting properties
  • Enhances emulsification
  • High purity product for research applications

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

235.4 °F - closed cup

Flash Point(C)

113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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J Fernandez et al.
Analytical biochemistry, 218(1), 112-117 (1994-04-01)
An improved and simplified procedure for enzymatic digestion of proteins bound to polyvinylidene difluoride (PVDF) membranes for obtaining internal protein sequence data is presented. This improved procedure is compatible with various enzymes (trypsin, endoproteinase Lys-C, endoproteinase Glu-C, and clostripain) and
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Rappon M and Lin SE
Journal of Molecular Liquids, 116(3), 125-130 (2005)
S J Milder et al.
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Time-resolved difference spectra have been obtained for the photocycle of delipidated bacteriorhodopsin monomers (d-BR) in six different detergent micelle environments that were prepared by two new detergent-exchange techniques. A global kinetic analysis of the photocycle spectra for d-BR in each
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