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93422

Sigma-Aldrich

Triton™ X-114

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Synonym(s):
(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
(C2H4O)n C14H22O, n = 7 or 8
CAS Number:
9036-19-5
Beilstein:
2315025
MDL number:
MFCD00128254
PubChem Substance ID:
57653556
NACRES:
NA.21

description

non-ionic

Quality Level

200

form

liquid

mol wt

average mol wt 537

impurities

≤0.001% peroxides (as H2O2)
≤1% water

ign. residue

≤0.2%

refractive index

n20/D 1.488 (lit.)
n20/D 1.495

CMC

0.2 mM (20-25°C)

transition temp

pour point ~−9 °C
cloud point 23 °C

density

1.052 g/mL at 20 °C
1.058 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤15 mg/kg
K: ≤500 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤500 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

HLB

12.4

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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1 of 4

This Item
X100X11493443
Triton™ X-114

Sigma-Aldrich

93422

Triton™ X-114

Triton™ X-100 laboratory grade

Sigma-Aldrich

X100

Triton™ X-100

Triton™ X-114 laboratory grade

Sigma-Aldrich

X114

Triton™ X-114

Triton™ X-100 solution BioUltra, for molecular biology, ~10% in H2O

Sigma-Aldrich

93443

Triton™ X-100 solution

form

liquid

form

liquid

form

-

form

liquid

mol wt

average mol wt 537

mol wt

micellar avg mol wt 80,000, average mol wt 625

mol wt

average mol wt 537

mol wt

micellar avg mol wt 80,000, average mol wt 625

impurities

≤0.001% peroxides (as H2O2), ≤1% water

impurities

-

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

CMC

0.2 mM (20-25°C)

CMC

0.2-0.9 mM (20-25°C)

CMC

0.2 mM (20-25°C)

CMC

0.2-0.9 mM (20-25°C), 0.23 mM

transition temp

pour point ~−9 °C, cloud point 23 °C

transition temp

cloud point 65 °C, pour point ~7 °C

transition temp

pour point ~−9 °C, cloud point 23 °C

transition temp

cloud point 65 °C, pour point ~7 °C

General description

Triton™ X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton™ X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton™ X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton™ X-114 has been used:
  • In phase separation of integral membrane proteins
  • In the purification of bacterial inclusion bodies
  • To study the clouding phenomenon in promethazine hydrochloride, an amphiphilic phenothiazine drug
  • To partition conditioned media into insoluble and soluble phases before Western blotting
  • For solubilization of phosphatidylinositol 4-phosphate 5-kinase from bovine brain

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word

Danger

Hazard Statements

H302 - H315 - H318 - H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Triton™ X-100 laboratory grade

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Potassium chloride tested according to Ph. Eur.

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282103

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Phospholipase C from Clostridium perfringens (C. welchii) Type I, lyophilized powder, 10-50 units/mg protein

Sigma-Aldrich

P7633

Phospholipase C from Clostridium perfringens (C. welchii)

Triton™ X-100 for analysis

Millipore

1.08603

Triton™ X-100

Phospholipase C from Bacillus cereus ≥200 units/mg protein

Sigma-Aldrich

P6621

Phospholipase C from Bacillus cereus

A Sánchez-Ferrer et al.
Plant physiology, 91(4), 1481-1487 (1989-12-01)
Polyphenoloxidase from grape berries is extracted only by nonionic detergents with a hydrophilic-lipophilic balance between 12.4 and 13.5. The enzyme was partially purified in latent form, free of phenolics and chlorophylls, by using temperature phase partitioning in a solution of
J.G. Pryde
Trends in Biochemical Sciences, 11, 160-160 (1986)
Expression, purification and immunological characterization of the transforming protein E7, from cervical cancer-associated human papillomavirus type 16.
Fernando G J P, et al.
Clinical and Experimental Immunology, 115(3), 397-397 (1999)
Identification of a new 80 k isoform of phosphatidylinositol 4-phosphate 5-kinase from bovine brain.
Schmidt M, et al.
Febs Letters, 325(3), 201-204 (1993)
M Schmidt et al.
FEBS letters, 325(3), 201-204 (1993-07-05)
Phosphatidylinositol 4-phosphate 5-kinase is associated with bovine brain microsomes to an extent of approximately 65% of the total cellular enzyme activity. This membrane-associated kinase activity can be solubilized with Triton X-114. Following polyacrylamide gel electrophoresis in the presence of SDS
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