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E4274

Sigma-Aldrich

Endotoxin Removal solution

Remove lipopolysaccharides (LPS) from plasmid DNA preparations

Synonym(s):

ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal reagent, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.52

Quality Level

200

Application

Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker′s yeast RNA.

Biochem/physiol Actions

The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.

Quantity

1 volume treats 5 volumes of DNA solution (≤1mg/ml)

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Determination of endotoxin through an aptamer-based impedance biosensor.
Su W
Biosensors And Bioelectronics, 32(1), 32-36 (2012)
M Cotten et al.
Gene therapy, 1(4), 239-246 (1994-07-01)
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)

Articles

Cell Culture FAQs: Bacterial Endotoxin Contamination

FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.

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