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S3014

Sigma-Aldrich

Sodium chloride

for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Synonym(s):

Halite, NaCl

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About This Item

Linear Formula:
NaCl
CAS Number:
7647-14-5
Molecular Weight:
58.44
Beilstein/REAXYS Number:
3534976
EC Number:
231-598-3
MDL number:
MFCD00003477
UNSPSC Code:
12352302
PubChem Substance ID:
24899542
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

assay

≥99% (titration)

form

crystalline

impurities

DNAse, none detected
 Endonuclease, none detected
 NICKase, none detected
 RNAse, none detected
 protease, none detected

turbidity

≤3.5 NTU

pH

7

mp

801 °C (lit.)

solubility

H2O: 1 g/10 mL, clear, colorless

density

2.16 g/cm3 at 25 °C (77 °F)

anion traces

phosphate (PO43-): ≤5 ppm

cation traces

K: ≤50 ppm
≤5 ppm (Heavy Metals by ICP-OES)

suitability

suitable for Western blot
suitable for mass spectrometry
suitable for molecular biology

application(s)

cell analysis

foreign activity

DNase, RNase, and protease, none detected

SMILES string

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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Application

This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the absence of nucleases and proteases.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Certificates of Analysis (COA)

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Jules D P Valentin et al.
Journal of colloid and interface science, 552, 247-257 (2019-05-28)
Many materials used in the medical settings such as catheters and contact lenses as well as most biological tissues are not purely elastic, but rather viscoelastic. While substrate elasticity has been investigated for its influence on bacterial adhesion, the impact
Zlatko Kopecki et al.
Oncotarget, 6(34), 36426-36440 (2015-10-27)
Flightless I (Flii) is an actin remodeling protein that affects cellular processes including adhesion, proliferation and migration. In order to determine the role of Flii during carcinogenesis, squamous cell carcinomas (SCCs) were induced in Flii heterozygous (Flii+/-), wild-type and Flii
Ram S Puranam et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(23), 8866-8881 (2015-06-13)
We identified a family in which a translocation between chromosomes X and 14 was associated with cognitive impairment and a complex genetic disorder termed "Genetic Epilepsy and Febrile Seizures Plus" (GEFS(+)). We demonstrate that the breakpoint on the X chromosome
Qian Chen et al.
Genes & development, 29(12), 1285-1297 (2015-06-26)
The Hippo signaling pathway converges on YAP to regulate growth, differentiation, and regeneration. Previous studies with overexpressed proteins have shown that YAP is phosphorylated by its upstream kinase, Lats1/2, on multiple sites, including an evolutionarily conserved 14-3-3-binding site whose phosphorylation
Viktor Posse et al.
Nucleic acids research, 43(5), 2615-2624 (2015-02-19)
A single-subunit RNA polymerase, POLRMT, transcribes the mitochondrial genome in human cells. Recently, a factor termed as the mitochondrial transcription elongation factor, TEFM, was shown to stimulate transcription elongation in vivo, but its effect in vitro was relatively modest. In
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Protocols

Annealing Oligonucleotides Protocol

Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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